27 Oct A pregnant 23-year-old healthy Saudi woman was admitted to the OB/GYN in the hospital for routine prenatal care at 12 weeks.

A pregnant 23-year-old healthy Saudi woman was admitted to the OB/GYN in the

hospital for routine prenatal care at 12 weeks. Her physician has ordered the ABO

typing and DAT, Antibody screen and Antibody identification. Your reference lab

technician has already called the woman’s OB/GYN physician, who reports that her

patient has never before been transfused and this is her first pregnancy. Her results

are as follows:

ABO/Rh type

Forward

Reverse

Anti-A

Anti-B

Anti-D

A, Cells

B Cells

4+

0

0

0

3+

A DAT was performed, with the following results:

Poly-specificanti-IgG/C3d:

Mono-specific anti-lgG:

Mono-specific anti-C3d:

Auto-control:

3+

4+

0

4+

Upon completion of the procedure the following results were obtained with the

Anti-human Globulin test, the laboratory technician has performed the antibody

screen with results as presented below:

Screening Cells

IS phase

37°C Phase

AHG Phase

3+

3+

Comb’s Control Cells

(Check Cells: CC)

3+

2+

I

3+

2+

3+

0

AC

0

1. What is the blood group type of the pregnant woman? What is the test you

should perform to confirm her Rh test result?

2. Interpret the DAT test result. What does the DAT mean in this case?

3. Answer the following questions regarding the antibody screening.

A. What Ab class(es) were present in the patient’s serum? Explain your answer.

B. Explain why the result indicates that there are multiple antibody specificities in

the patient’s serum?

C. Is there an autoantibody present in the patient’s serum? If so, which one?

Explain your answer.

D. Is your answer to C. clinically significant? Explain your answer. 

A pregnant 23-year-old healthy Saudi woman was admitted to the OB/GYN in the hospital for routine prenatal care at 12 weeks. Her physician has ordered the ABO typing and DAT, Antibody screen and Antibody identification. Your reference lab technician has already called the woman’s OB/GYN physician, who reports that her patient has never before been transfused and this is her first pregnancy. Her results are as follows: ABO/Rh type Forward Reverse Anti-A Anti-B Anti-D Al Cells B Cells 4+ 3+ A DAT was performed, with the following results: Poly-specific anti-IgG/C3d: 3+ Mono-specific anti-IgG: Mono-specific anti-C3d: Auto-control: 4+ Upon completion of the procedure the following results were obtained with the Anti-human Globulin test, the laboratory technician has performed the antibody screen with results as presented below: Screening Cells IS phase 37.C Phase AHG Phase Coomb’s Control Cells (Check Cells; CC) 3+ 2+ 3+ II 3+ 2+ 3+ III 3+ AC 1. What is the blood group type of the pregnant woman? What is the test you should perform to confirm her Rh test result? (1 Mark) 2. Interpret the DAT test result. What does the DAT mean in this case? (1 Mark) 3. Answer the following questions regarding the antibody screening. A. What Ab class(es) were present in the patient’s serum? Explain your answer. (1 Mark) B. Explain why the result indicates that there are multiple antibody specificities in the patient’s serum? (0.5 Mark) C. Is there an autoantibody present in the patient’s serum? If so, which one? Explain your answer. (1 Marl D. Is your answer to C. clinically significant? Explain your answer.