Question number [point value]

1.[3] Outline the critical features of the three phases of transcription. Explain how each can be a good target to regulate gene expression? Which of the three phases is the most common target of regulatory mechanisms.(MLO 5.1)

2.[3]Why does transcription not have to be as accurate as replication? Explain. (MLO 5.2)

3.[4] What changes do the promoter and the polymerase undergo during the transition from a closed to an open complex? What drives this transition in prokaryotes, and in eukaryotes? What is the importance of the transition for the overall transcription process?(MLO 5.2)

4.[3] Promoter clearance of RNA polymerase involves an inherent problem, in that the energetic forces that attract the polymerase to the promoter in the first place will tend to hold it there, at the promoter, thereby preventing transcription. How can the transcription machinery overcome this problem and enable the polymerase to clear the promoter? Be sure to explain both prokaryotic and eukaryotic mechanisms(MLO 5.2)

5.[4]  Name three ways in which mRNA is processed prior to being exported from the nucleus. How are these processing events coordinated with elongation and termination of transcription?(MLO 5.3)

6.[3] What is the function of the prokaryotic ?? factor?.(MLO 5.2)

7.[10] Describe the detailed processes involved in prokaryotic transcription a)initiation, b)elongation, and c) termination.(MLO 5.4)

8.[10] Describe the detailed processes involved in eukaryotic transcription a)initiation, b)elongation, and c) termination.(MLO 5.4)